Anti-ZO1 tight junction protein antibody （ab59720）抗ZO1緊密連接蛋白抗體

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Anti-ZO1 tight junction protein antibody (ab59720)抗ZO1緊密連接蛋白抗體產品信息：

• Product name：Anti-ZO1 tight junction protein antibodySee all ZO1 tight junction protein primary antibodies ...
• Description

  Rabbit polyclonal to ZO1 tight junction protein

• Specificityab59720 recognises both ZO1 tight junction alpha-minus and alpha-plus isoforms. The protein is abundant in endothelial cells and the highly specialized epithelial junctions. Although we have an image for IHC-Fr, customers report variable results in this application. If you would like to use in IHC-Fr then we suggest using ethanol instead of acetone. Although we have references for IHC-P, customers report variable results in this application. IHC-Fr and IHC-P are therefore not covered by our Abpromise guarantee.
• Tested applicationsICC/IF, Flow Cyt, IHC (PFA fixed), WB, IHC-P more details
• Species reactivity

  Reacts with: Mouse, Chicken, Dog, Human, Pig

• Immunogen

  Corresponding to amino acids 432-1150 of Human ZO1 tight junction protein

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.1% BSA, PBS

• Concentration 50 µg at 0.1 mg/ml

• Purification notes0.2µm filtered.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Tags & Cell Markers
  • Subcellular Markers
  • Plasma Membrane

Applications

Our Abpromise guarantee covers the use of ab59720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionThe N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.
• Tissue specificityThe alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
• Sequence similaritiesBelongs to the MAGUK family.
  Contains 1 guanylate kinase-like domain.
  Contains 3 PDZ (DHR) domains.
  Contains 1 SH3 domain.
  Contains 1 ZU5 domain.
• DomainThe second PDZ domain mediates interaction with GJA12.
• Post-translational
  modificationsPhosphorylated. Dephosphorylated by PTPRJ.
• Cellular localizationCell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.
• Information by UniProt

• Database links

  • Entrez Gene: 7082 Human
  • Entrez Gene: 21872 Mouse
  • Omim: 601009 Human
  • SwissProt: Q07157 Human
  • SwissProt: P39447 Mouse
  • Unigene: 510833 Human
  • Unigene: 4342 Mouse

• Alternative names

  • Tight junction protein 1 antibody
  • Tight junction protein ZO-1 antibody
  • Tight junction protein ZO1 antibody

Anti-ZO1 tight junction protein antibody images

• Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)

  ab59720 staining ZO1 in Caco-2 cells treated with (±)-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of (±)-palmitoylcarnitine chloride, as described in literature.
  The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141122 ((±)-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

• 

  Immunocytochemistry/ Immunofluorescence - ZO1 tight junction protein antibody (ab59720)

  ICC/IF image of ab59720 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)This image is courtesy of an anonymous Abreview

  Immunofluorescence analysis of HUVEC cells, staining ZO1 tight junction protein with ab59720.
  Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ZO1 tight junction protein antibody (ab59720)Image from Kokkinopoulos I et al., PLoS One. 2011 Apr 22;6(4):e18921. Fig 6.; doi:10.1371/journal.pone.0018921; April 22, 2011, PLoS ONE 6(4): e18921.

  Immunofluorescence analysis of murine peripheral retinal pigmented epithelium (RPE) cells, staining ZO1 tight junction protein with ab59720.
  
  Cells were fixed with 4% paraformaldehyde for 10 minutes at room temperature. Cells were blocked in 10% BSA and 0.5% Triton-X 100 for 2 hours at room temperature before being incubated with primary antibody (1/100) in blocking solution overnight at room temperature. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody.

公司介紹：

    Abcam位于英國的劍橋科學園，成立于1998年，專門生產和分銷研究型抗體。主要創立人Jonathan Milner和實驗室同事構思成立一間了解科研人員需要的網上抗體公司，理念是Abcam銷售質量高的抗體，并提供zui全面、誠實、時新的說明書，快速的送貨服務，及高效率的顧客和服務。我們的在線目錄(www.abcam.cn) 已有差不多100,000種抗體和試劑，并不斷添加，供應予百多個國家。Abcam于2005年11月在倫敦證券交易所上市，在美國馬薩諸塞州、日本東京及香港均設有分公司。

    Abcam 一直致力加強產品線，為使研究員更容易找到蛋白質研究試劑產品，在2011并購了美國的MitoSciences公司，加強了免疫分析方面的產品供應；同年也并購了英國的Ascent Scientific 公司，開展了生化試劑的供應。在2012年，Abcam并購了美國的Epitomics 公司，成為一家有的RabMAbs® 供應商。

    Abcam 的 目標是給世界上的抗體建立zui大的在線目錄，為各地科學家提供*產品，成為各國科學界的重要伙伴。我們為所有產品提供來使客戶獲得預期的結 果。為提供高質量的抗體來指向盡可能多的靶蛋白，我們盡所能在盡可能多的應用和物種中檢測每種抗體。我們相信誠信才是上策，在我們的上會發布有關我們 每一種產品的盡可能多的信息。

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