Anti-Nrf2 antibody （ab31163）抗核轉錄因子2抗體

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Anti-Nrf2 antibody (ab31163)抗核轉錄因子2抗體產品信息：

• Product nameAnti-Nrf2 antibodySee all Nrf2 primary antibodies ...
• Description

  Rabbit polyclonal to Nrf2

• Specificityab31163 recognises Nrf2 protein
• Tested applicationsIHC-P, ICC/IF, IHC-Fr, WB more details
• Species reactivity

  Reacts with: Mouse, Rat, Human
  Predicted to work with: Chicken, Cow

• Immunogen

  Synthetic peptide: TL YLEVFSMLRD EDGKPYSP, corresponding to amino acids 569-588 of Human Nrf2

• Positive control
  • Placenta

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 1% BSA, 10mM PBS, pH 7.4

• Concentration 500 µl at 0.2 mg/ml

• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors
  • Cell Biology
  • Other Antibodies
  • Oxidative Stress
  • Cardiovascular
  • Heart
  • Cardiac metabolism
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial Biogenesis
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism
  • Molecular processes
  • Mitochondrial transcription
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

Applications

Our Abpromise guarantee covers the use of ab31163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionTranscription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
• Tissue specificityWidely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
• Sequence similaritiesBelongs to the bZIP family. CNC subfamily.
  Contains 1 bZIP domain.
• DomainAcidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
• Post-translational
  modificationsPhosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
• Cellular localizationCytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
• Information by UniProt

• Database links

  • Entrez Gene: 396014 Chicken
  • Entrez Gene: 497024 Cow
  • Entrez Gene: 4780 Human
  • Entrez Gene: 18024 Mouse
  • Entrez Gene: 83619 Rat
  • Omim: 600492 Human
  • SwissProt: Q5NUA6 Cow
  • SwissProt: Q16236 Human

• Alternative names

  • erythroid derived 2 antibody
  • HEBP1 antibody
  • like 2 antibody

Anti-Nrf2 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 antibody (ab31163)

  ab31163 at 1/100 dilution, staining Nrf2 in human placenta by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

• 

  Immunocytochemistry/ Immunofluorescence-Nrf2 antibody(ab31163)

  ICC/IF image of ab31163 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31163, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Western blot - Nrf2 antibody (ab31163)

  All lanes : Anti-Nrf2 antibody (ab31163) at 1 µg/ml
  Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
  Lysates/proteins at 10 µg per lane.
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  Performed under reducing conditions.
  Predicted band size : 68 kDa
  Observed band size : 68 kDa
  Additional bands at : 28 kDa,37 kDa. We are unsure as to the identity of these extra bands.
  Exposure time : 1 minute

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