Anti-hnRNP K antibody EP943Y （ab52600）抗核糖核蛋白K抗體

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Anti-hnRNP K antibody [EP943Y] (ab52600)抗核糖核蛋白K抗體產品信息：

• Product nameAnti-hnRNP K antibody [EP943Y]
• Description

  Rabbit monoclonal [EP943Y] to hnRNP K

• SpecificityThis antibody detects both phosphorylated and unphosphorylated hnRNP K.
• Tested applicationsWB, IP, IHC-P, ICC/IF
• Species reactivity

  Reacts with: Mouse, Rat, Human

• Immunogen

  A synthetic peptide corresponding to residues near the N-terminus of human hnRNP.

• Positive control
  • Jurkat cell lysate; HeLa cells; Human breast carcinoma tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP943Y
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Other

Applications

Our Abpromise guarantee covers the use of ab52600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

• FunctionOne of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA.
• Sequence similaritiesContains 3 KH domains.
• Post-translational
  modificationsArg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.
• Cellular localizationCytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear.

• Database links

  • Entrez Gene: 3190 Human
  • Entrez Gene: 15387 Mouse
  • Entrez Gene: 117282 Rat
  • Omim: 600712 Human
  • SwissProt: P61978 Human
  • SwissProt: P61979 Mouse
  • SwissProt: P61980 Rat
  • Unigene: 522257 Human

  see all

• Alternative names

  • CSBP antibody
  • dC stretch binding protein antibody
  • FLJ41122 antibody

  see all

Anti-hnRNP K antibody [EP943Y] images

• 

  Western blot - hnRNP K antibody [EP943Y] (ab52600)

  Anti-hnRNP K antibody [EP943Y] (ab52600) at 1/100000 dilution + Jurkat cell lysate at 10 µg
  
  Secondary
  HRP-labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 51 kDa
  Observed band size : ~60 kDa (why is the actual band size different from the predicted?)

• 

  Immunohistochemistry (Paraffin-embedded sections) - hnRNP K antibody [EP943Y] (ab52600)

  Immunohistochemical staining of paraffin-embedded human breast carcinoma tissue using ab52600 at a dilution of 1/100-1/250.

• 

  Immunocytochemistry/ Immunofluorescence - hnRNP K antibody [EP943Y] (ab52600)

  Fluorescent immunostaining of HeLa cells using ab52600 at a dilution of 1/100-1/250.

公司介紹：

    Abcam位于英國的劍橋科學園，成立于1998年，專門生產和分銷研究型抗體。主要創立人Jonathan Milner和實驗室同事構思成立一間了解科研人員需要的網上抗體公司，理念是Abcam銷售質量高的抗體，并提供zui全面、誠實、時新的說明書，快速的送貨服務，及高效率的顧客和服務。我們的在線目錄(www.zshare.cc) 已有差不多100,000種抗體和試劑，并不斷添加，供應予百多個國家。Abcam于2005年11月在倫敦證券交易所上市，在美國馬薩諸塞州、日本東京及香港均設有分公司。

    Abcam 一直致力加強產品線，為使研究員更容易找到蛋白質研究試劑產品，在2011并購了美國的MitoSciences公司，加強了免疫分析方面的產品供應；同年也并購了英國的Ascent Scientific 公司，開展了生化試劑的供應。在2012年，Abcam并購了美國的Epitomics 公司，成為一家有的RabMAbs® 供應商。

    Abcam 的 目標是給世界上的抗體建立zui大的在線目錄，為各地科學家提供*產品，成為各國科學界的重要伙伴。我們為所有產品提供來使客戶獲得預期的結 果。為提供高質量的抗體來指向盡可能多的靶蛋白，我們盡所能在盡可能多的應用和物種中檢測每種抗體。我們相信誠信才是上策，在我們的上會發布有關我們 每一種產品的盡可能多的信息。

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